Chromophore-assisted laser inactivation (CALI) is a method of achieving acute and localized knockdown of protein function by activation of photosensitizers directed to a particular target.

This approach is unique in its capacity to facilitate rapid and specific inactivation of proteins in cells and in vivo. Optical control over cellular function provided by CALI, in combination with genetically-encoded photosensitizers, has led to the development of optogenetics.

CALI-derived technologies
CALI-derived technologies. (Hoffman-Kim D, et al. Methods Cell Biol. 2007.