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ISPR array. The cell uses the CRISPR elements to distinguish between genomic and invading DNA (Marraffini & Sontheimer, 2010). Next a CRISPR unit is transcribed to a CRISPR RNA (crRNA) and the crRNA is joined with a transactivating CRISPR RNA (tracrRNA) to form guide RNA (gRNA). The gRNA is bound by the Cas9 protein and causes the enzyme to undergo a conformational change, which activates the two nuclease domai
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