Applications

 

We’ve seen how Crispr/Cas9 systems can very easily be leveraged to achieve highly selective cleavage of double stranded DNA at a higher efficiency than previous technologies such as zinc finger nucleases or TALENs.  And we’ve seen a few pathways where the technology is adapted for different types of genome-editing and modification.  But the Crispr/Cas9 system can also be used to directly translate to new therapies and cures for diseases, and has been shown to have potential as a cure for HIV.  Several delivery methods are under investigation.

 

20 Responses to Applications

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    ISPR array. The cell uses the CRISPR elements to distinguish between genomic and invading DNA (Marraffini & Sontheimer, 2010). Next a CRISPR unit is transcribed to a CRISPR RNA (crRNA) and the crRNA is joined with a transactivating CRISPR RNA (tracrRNA) to form guide RNA (gRNA). The gRNA is bound by the Cas9 protein and causes the enzyme to undergo a conformational change, which activates the two nuclease domai

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