CRISPR: Prokaryotic Adaptive Immune System

(original figure)

The CRISPR-Cas9 system defends against phage and conjugative plasmid infection. First the cell incorporates the invading DNA into a CRISPR array. The cell uses the CRISPR elements to distinguish between genomic and invading DNA (Marraffini & Sontheimer, 2010). Next a CRISPR unit is transcribed to a CRISPR RNA (crRNA) and the crRNA is joined with a transactivating CRISPR RNA (tracrRNA) to form guide RNA (gRNA). The gRNA is bound by the Cas9 protein and causes  the enzyme to undergo a conformational change, which activates the two nuclease domains. This enzyme-RNA complex then catalyzes a double-strand break in the target DNA.

In the bacterial immune system, this process inhibits the viral infection. Researchers, however, can use this process to target and modify specific regions of host DNA.

 

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  252. nongnee says:

    In one of his last pictures Siddiqui photographed a member of Afghan special forces firing at Taliban fighters at a check post in Afghanistan’s Kandahar province. Siddiqui was embedded as a journalist since earlier this week with Afghan special forces in Kandahar
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  253. nongnee says:

    In a statement OnlyFans said the account did not have two-factor authentication, which made it vulnerable. The company said Tina did not report the racial slur and it was not detected by the site’s moderation system because it was pluralised.
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  254. angela1995 says:

    The government’s language too has changed significantly, from not saying much in the early days of the lawlessness to calling out what it says has been “economic sabotage”. Some commentators have even gone as far as to warn that the unrest has the hallmarks of an “attempted coup” and an “insurrection”.
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  255. nongnee says:

    As the virus ravaged Brazil the 32-year-old had spent more than a year risking his life, working as an Uber driver. Diabetes and high blood pressure made Mr Cruz especially vulnerable. But he kept driving passengers across São Paulo to make ends meet.
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  256. nongnee says:

    Unlike some other countries  Brazil chose not to hold back supplies for second doses. Its vaccination campaign has mostly relied on CoronaVac shots, made locally using inputs from China’s Sinovac. But shipments of Chinese materials have lagged, just as millions are due for their second jab.
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  257. dofin155 says:

    When questioned on Ms Hopkins  entry Ms Andrews said the decision had been made by New South Wales state government “on the basis of potential benefit to the economy”
    But opponents accused the federal government of “allowing a far-right troll into Australia”, noting Ms Hopkins had also been detained in South Africa in 2018 for spreading racial hate.
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  258. nongnee says:

    Prof Daniel Mills a veterinary behavioural specialist from the University of Lincoln, who has seen the footage, said: “A gunshot going off is going to be startling, seeing another horse suddenly drop, these are all going to be very distressing for a horse in this situation.
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  259. nongnee says:

    Despite claiming during his first year in office that Islamist militant group Boko Haram had been “technically” defeated, President Buhari now admits that his government is failing to stop the insurgency, which began in the north-east.
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  260. jusmin says:

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  261. momo says:

    Western security sources believe Hafnium obtained advance knowledge that Microsoft intended to patch or close the vulnerability, and so shared it with other China-based groups to maximise the benefit before it became obsolete.
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  262. dofin155 says:

    That May she was detained by Saudi authorities along with a number of other women activists as part of an apparent crackdown on dissent overseen by Crown Prince Mohammed bin Salman.
    Ms Hathloul was convicted last December of seeking to change the political system and sentenced to six years in prison.
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  263. dofin155 says:

    Mr. Varadarajan according to media reports, is one of the activists, journalists, politicians and lawyers around the world that have been targeted with phone spyware sold to governments by an Israeli firm.
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  264. angela1995 says:

    The latest episode, according to Ms. Lakshane points to the “scale and extent of electronic surveillance by the government and the lack of safeguards against such snooping”. India, she said, was in “dire need of surveillance reform”.
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  265. angela1995 says:

    Regardless of whether Republicans or Democrats are in charge, nearly all US cities have seen a rise in gun violence and killings. Whether local police funding has increased or decreased has not spared them.
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  266. Video And Animation Services is a method in which figures are manipulated to appear as moving images. Television and video are popular electronic animation media that originally were analog and now operate digitally.

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  268. angela1995 says:

    From Wednesday, people need proof of vaccination, a negative test or recent recovery from Covid to enter most museums and cinemas. Later lawmakers will debate whether to extend its use in August for entry to cafes, restaurants and shopping malls. It comes the day after Health Minister Olivier Véran announced 18,000 new infections in just 24 hours.
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  269. hooly124 says:

    Popular author and columnist Shobhaa De wrote that the image of “a grief-stricken Mandira doing her husband’s last rites challenged archaic norms governing our society for centuries and sent out a charged and powerful anti-patriarchy message”.
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  270. nongnee says:

    A few months later the pandemic had engulfed Mexico and thousands of people were dying every week. But Coquilteel and many small, indigenous towns in the state of Chiapas were left relatively unscathed. This has been a blessing but it also presents a problem.
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  271. banana says:

    The absence of joint UN African Union peacekeepers is being felt by many in West Darfur. After 13 years on the ground, experts say their gradual withdrawal since December has been met with a surge of violence.
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  272. dofin155 says:

    The United States women gymnastics team arrived at Atlanta 1996 as the Magnificent Seven – a stellar group who could finally challenge the Russian team who, in different guises, had won the last two all-around titles.
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  273. rose says:

    According to local media reports residents living near the Tielu Railway Station also delivered food and bottled water to passengers stuck in a train after the hashtag “K226 call for emergency rescue” was viewed more than 800 million times.
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  274. roling says:

    Daley is Britains most decorated diver, but he possesses a level of fame that far transcends his discipline. He has become one of sport’s highest-profile gay athletes, a role model for others in the LGBTQ+ community. He has suffered several media run-ins during the course of a life lived under the spotlight.
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  275. sdrtrh says:

    Campbell mother was a constant source of strength and inspiration to her son. She was a strict parent – Campbell describes her as “the most feared on the estate” – but the fact she often worked two or three jobs meant he also spent plenty of time with his extended group of friends, for whom trouble was never far away.
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  276. saiyong says:

    Sampras crashes a serve out wide and begins a charge towards the net, he wants to follow up with a signature volley. But the return is too powerful and beyond his reach as it comes thumping down the line. It all happens so quickly. The crowd roars as the 19-year-old winner falls to the ground in disbelief, a five-set epic over in an instant. He has beaten the champion, his idol.
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  277. saiyong says:

    It was only ever going to be a matter of time before he moved into coaching. For five years, he had shared the Blaugrana changing room with Pep Guardiola. They had a special connection and would talk football for hours, conversations that helped form their vision of an evolved style based on Johan Cruyff’s Barcelona.
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  278. momo says:

    The films were shot in remote locations – bungalows rented for the day in the suburbs of Mumbai. “New and aspiring female actors were lured by the accused, promising them roles in short films and web series,” said Mumbai joint police commissioner for crime, Milind Bharambe.
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  279. nongnee says:

    Their driveway has gravel that drains water, rather than collecting it into pools, and their green roof reduces rainwater run-off. And under their rear garden is a soakaway: essentially a big tank constructed of crates that collects excess rainwater and gradually drains it into the subsoil.
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  280. dofin155 says:

    They say they funded a project to examine “at the molecular level” newly-discovered bat viruses and their spike proteins (which help the virus bind to living cells) “without affecting the environment or development or physiological state of the organism”.
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  281. dofin155 says:

    In her consulting room, the doctor sees the evidence of semi-contractual, often temporary liaisons between men and women. These are personal relationships in Sinaloa shaped – some would say misshaped – by drug trafficking.
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  282. nongnee says:

    When the Games were postponed in March 2020, organisers said the Olympic flame “could become the light at the end of the tunnel”. With the Covid-19 pandemic still raging worldwide, that metaphorical tunnel is still being traversed, but Friday’s opening ceremony offers a glimmer of that light.
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  283. Very good and weighty article, I hope you can share information like this again, I will be happy to read and share your article. daftar slot pulsa

  284. nongnee says:

    Last week the WFP suspended movement of convoys from Afar into Tigray after a convoy of 10 vehicles was attacked. It’s not clear who carried out the attack, but observers suggest pro-government militia were responsible. A further convoy of more than 200 trucks remains on standby in the Afar town of Semera, but the WFP says the trucks won’t leave for Tigray until the security situation improves.
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  285. dofin155 says:

    New proposals are approved or rejected by a group known as the Emoji Subcommittee, part of the Unicode Consortium, a not-for-profit organisation made up of representatives from most of the world’s biggest tech firms including Google, Microsoft, Adobe and Huawei.
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  286. nongnee says:

    Haitian police accuse a group made up of 26 Colombians and two Haitian Americans of carrying out the killing. At least 20 were detained, while three were killed by police and five are still on the run.
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  287. dofin155 says:

    There have also been problems with a specialist ingredient called lipid nanoparticles – ultra-tiny particles that are crucial to the pioneering mRNA vaccines produced by BioNTech/Pfizer and Moderna.
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  288. dofin155 says:

    Mr Bezos and the three other crew members who flew on Blue Origin’s spacecraft may have less claim to the coveted title. Ahead of the launch, Blue Origin CEO Bob Smith said that “there’s really nothing for a crew member to do” on the autonomous vehicle.
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  293. nongnee says:

    The process usually took up to two decades back then – a government-owned monopoly was the sole source of phone connections. By the mid-1980s, nearly a million people were waiting for a phone.
    Before July 1991 – when historic reforms threw open the door to India’s economy – such “consultants” roamed outside offices in the country, offering out-of-turn phone connections, driving licences and passports for a hefty premium.
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  294. nongnee says:

    Mr Zikalala had earlier faced criticism for suggesting that in order to calm the situation, the authorities should release former President Jacob Zuma from prison.
    It was Zuma’s arrest for contempt of court which sparked the unrest leading to claims that his allies were seeking to overthrow South Africa’s young democracy.
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  295. momo says:

    The third of nine children, Mr Castillo was born in Puña in 19 October 1969, and worked as a primary school teacher from 1995 to 2020. He started his political career in 2002, when he unsuccessfully ran for mayor, and first rose to prominence in 2017 during a teachers’ strike over pay and performance evaluation.
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  296. nongnee says:

    These passports are usually accessed via an app on your phone or in some cases, paper versions are available.
    Some nations have already installed their own versions of a Covid passport which has allowed people to attend large scale events. But with some countries lagging behind with vaccinations, there is concern that some could be left behind.
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  297. momo says:

    Britain’s seventh gold in Tokyo came after Yee pulled clear in the 2km run having been briefly overtaken in the bike stage by France’s Vincent Luis.
    Britain finished 14 seconds ahead of the United States while France claimed the bronze medal.
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  298. momo says:

    The victory was all the more impressive given Dawson slipped off the wall at the start of her backstroke leg before fighting back superbly to give her team-mates the chance to chase down their opponents.
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  299. nongnee says:

    Peaty and Guy overturned the deficit in the middle two splits – the latter completing his 100m in just 50 seconds – before Hopkin held on in a final 100m that featured American superstar Caeleb Dressel.
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  300. nongnee says:

    The Nigerian – a rival to Great Britain’s Dina Asher-Smith – had won her 100m heat on Friday and was due to race in the semi-finals on Saturday.
    The Athletics Integrity Unit (AIU) said the 32-year-old had tested positive for a human growth hormone following an out-of-competition test on 19 July.
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  301. angela1995 says:

    She could barely stand after ditching her bike, moments after becoming a champion. Team-mate Kye Whyte, who himself had just won silver in the men’s event, swept her up in his arms, the pair deservedly milking their moment as they became Britain’s first BMX medallists.
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  302. nongnee says:

    Afterpay has been seen as a key indicator of the prospects for the niche no-credit-checks online payments industry that jumped in popularity last year as more users, especially young people, opted to pay in instalments for everyday items during the coronavirus pandemic.
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  303. nongnee says:

    Since the 1990s, the South Korean government has had to create policies to encourage Korean men, initially those in rural areas who could not find a match, to marry women from overseas.
    But the lives of these “marriage migrant” women are not easy. Some face stigma once they move to South Korea, and there have been reports of domestic violence and abuse.
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  304. nongnee says:

    a species of gum (eucalyptus) tree has seeds that are coated in a resin requiring them to be exposed to fire in order to melt the resin and expose the seed within. There are other trees with thick barks which act as heat shields to protect the vital sapwood that transports nutrients and water throughout the tree.
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  305. momo says:

    If Muir’s progression was straightforward, Hassan, who is due to compete in the 5,000m final later on Monday, was made to work in the final 300m so she could make up ground and take on the field after her fall.Ethiopia-born Hassan – who runs for the Netherlands after arriving in the country as a refugee aged 15 – is expected to challenge for a medal over 1500m, 5,000m and 10,000m in Tokyo.
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  306. dofin155 says:

    While the majority of Team GB bathed in the golden glory of the sun-soaked successes at the home Games, British swimmers were noticeable absentees from the podium party.
    Rebecca Adlington, who had claimed two Olympic gold medals at Beijing 2008, secured double bronze and Michael Jamieson impressed with silver, but the team fell short of their medal target, which was between five and seven medals.
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  307. saiyong says:

    Fourth in the world against second in the world. Joe Root versus Virat Kohli. England’s record-breaking seamers against India’s top-class spinners.
    England have been almost unbeatable on home soil – their series defeat by New Zealand in June was their first since 2014 – but India dished out a 3-1 hiding when they hosted Root’s side earlier this year.
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  308. fdrtgrh552 says:

    Fourth in the world against second in the world. Joe Root versus Virat Kohli. England’s record-breaking seamers against India’s top-class spinners.
    England have been almost unbeatable on home soil – their series defeat by New Zealand in June was their first since 2014 – but India dished out a 3-1 hiding when they hosted Root’s side earlier this year.
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  309. saiyong says:

    Lining up bottom of the leaderboard going into her second run, a conservative, bronze-medal routine may have been the sensible approach. But Worthington and coach Jamie Bestwick, a trailblazer in BMX freestyle, had other ideas.”I’ve learned if you gamble and give yourself that chance then it can pay off and you’ll feel better than if you hold back,” she said.
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  310. kongko152 says:

    Weightlifter Campbell had won European gold earlier this year but was left “speechless” after adding an Olympic silver to her medal collection.
    Making her Olympic debut in Tokyo, the 27-year-old was fourth after the snatch but moved on to the podium with successive clean and jerk lifts of 156kg and 161kg.
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  311. lora says:

    The incident has again put the spotlight on Belarus, which has been ruled by President Alexander Lukashenko since 1994. Last year, nationwide protests over his disputed re-election were violently repressed by the security forces.
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  312. saiyong says:

    After a near 16-year period of the record remaining in tact, Muhammad and McLaughlin have now exchanged the world best on four occasions in the last two years.
    McLaughlin superbly judged her effort in chasing down Rio 2016 champion Muhammad, who set the early pace and still held a slim advantage over the final hurdle.
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  313. saiyong says:

    The Norwegian came into the race as the world record holder. Five weeks ago he took eight hundredths off one of track’s longest-standing marks, lowering Kevin Young’s 29-year-old record to 46.70 seconds.On that occasion, he sunk his face into his hands. This time he didn’t dare. Warholm, eyes shocked wide, kept glancing back at the clock, fearing there was an error, that the time would be revised, that he was still asleep in his cardboard bed back in the athletes’ village. But it stayed and stood.
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  314. saiyong says:

    Krystina Timanovskaya, 24, boarded a flight to Vienna on Wednesday morning. She had been expected to travel directly to Warsaw as Poland has granted her a humanitarian visa, but changed destination at the last minute, an airport official told reporters. She told the BBC her actions were not a political protest: “I love my country and I didn’t betray my country.”
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  315. saiyongs says:

    One clip shows nine Israeli baseball players jumping up and down on the same bed to test its cardboard frame. Another shows Australian teammates taping a sign to their balcony to flirt with the American athletes in a neighbouring tower. There are videos of basketballers learning how to put on a tie for the opening ceremony; sprinters trying out Japanese snacks; fencers making fun of their own slip-ups.
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  316. saiyong says:

    When Mr Ghosn arrived at Tokyo’s Haneda airport, however, he soon found himself under arrest. He was charged with financial crimes, including failing to report his true earnings. Prosecutors were acting on information provided by Nissan itself, much of it collated by Mr Ghosn’s own chief of staff Hari Nada.
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  317. nongnee says:

    Belarus says she was removed from the national team because of her emotional state. But speaking to the BBC on Tuesday, Ms Timanovskaya said she did not suffer from any mental health issues and had not had any conversations with doctors at the Olympic village.
    อ่านต่อเพิ่มเติม โรงเรียนวัดควนส้าน

  318. saiyongs says:

    The International Olympic Committee (IOC) has launched a formal investigation into allegations that Belarus attempted to force her to return home. It has also requested a report on the incident from the Belarus National Olympic Committee.
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  319. kongko152 says:

    Antonoff has a reputation for generosity and empathy. His laid-back studio demeanour is free of the sexist power games that can sour the relations between male producers and female artists.
    The 37-year-old consistently describes his collaborators as friends – even when it’s rock icon Bruce Springsteen.
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  320. nongnee says:

    During a normal summer, Glacier Bay and the surrounding area buzzes with traffic, as vessels of all sizes, from massive, 150,000-tonne cruise liners to smaller whale-watching boats, ply the waters as part of Southern Alaska’s massive tourism industry.
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  321. ladykaka says:

    living with a long-term condition, and numbers rising, it’s no surprise the chronically ill community has exploded online in the last few years. Celebrities like Lady Gaga, Selena Gomez and Lena Dunham are opening up about their conditions, and chronic illness influencers are attracting huge social media followings.
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  322. John Stones says:

    French football expert Julien Laurens added: “I still think Barca are bluffing. From a PSG point of view and what they have said over the last year is that if Messi becomes available, and is interested, then they believe they have got enough in bank and resources to make that deal happen.
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  323. John Stones says:

    The Englishman also had more carries of the ball (543) than any of his new team-mates and his 65 dribbles was a total bettered only by Sterling’s 66, but it is his ability to win free-kicks that may prove to be the biggest difference.
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  324. John Stones says:

    Grealish was fouled 110 times last term, more than any other Premier League player, and with De Bruyne and Mahrez potent on set-pieces, it could lead to more goalscoring opportunities for someone such as centre-back John Stones, who netted five times during the last campaign.
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  325. John Stones says:

    This kind of charming nonchalance is pretty consistent with the character who came across on the series – while the other villa residents tore one another’s hair out, he mostly just floated around in the pool, eating lollies, wearing nice hats, looking a bit bemused by all of the fuss.
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  326. John Stones says:

    As far back as you can trace the roots, people ate what was local, and what is local along the Nile is molokhia,” food historian-cum-food health writer Michelle Berriedale-Johnson said. To this day, 95% of Egyptians live along the fabled river’s life-giving banks and arc-shaped delta.
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  327. Thanks for the sharing.

  328. bobbie says:

    Going on a road trip along the coast of the oldest continent on Earth was bound to be steeped in mysteries. As I passed green and yellow road signs warning to keep a look out for kangaroos, emus and echidnas, there was another rare life form I was seeking an audience with – one that traces its ancestry to the beginning of time.
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  329. bobbie says:

    The greater glider is the only member of the ringtail possum family that doesn’t have a grippy prehensile tail. But it is also unique from its relatives in that it dines exclusively on eucalyptus leaves (like koalas) and has gliding membranes that run only from its elbows to its ankles (unlike its cousins, which have membranes stretching to their forepaws). This allows them to perform more controlled glides and gives them something of a superhero quality in flight.
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  330. bobbie says:

    the holiday island’s elite properties are sandwiched between this rainforest and the north coast. Though these resorts are usually only open to guests, my guide, French primatologist and passionate conservationist , has forged relationships with the resident naturalists and we were granted access to wander
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  331. bobbie says:

    How far up a stream and inland each of the four species of climbing fish go seems to be related to their adult size. “The biggest ” said Grabowski – referring towhich can grow up to a foot in length – “may not get past the lower reaches of most streams.” As you head further inland and up the mountain, larger species peter out and the smaller fish dominate.
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  332. bobbie says:

    The stromatolites resemble cauliflower – big, pillow beige structures that grow upwards from the lagoon’s limestone bottom of the lake. They look like rocks, but they are actually living things. The sediment layers itself  with the aid of organisms called cyanobacteria, until the structures turn into a rocky underwater growth that can be seen on the surface of shallow water.
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  333. momo says:

    The sun sets on a Games like no other, and the Olympic family prepares to pack up and leave town, thoughts will now turn to how one of the most controversial sports events in modern history will be remembered in years to come. Every Games is different of course. But Tokyo 2020 was truly unprecedented. Regardless of the undoubted sporting drama that has unfolded here over the last 17 days, it will always be the Covid Olympics. The first such event to be staged since the start of the pandemic, the first to take place amid a state of emergency, and the only one to be stripped of spectators.
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  334. momo says:

    The United States top the medal table for the sixth time in seven Olympic Games. At all seven, they have won the most medals. Japan’s third place in the medal table – with 58 medals including 27 gold – is the country’s joint best with Tokyo 1964 when hosting and Mexico 1968.Great Britain finished second in Rio 2016 and third in London 2012. However, this is Team GB’s second best performance at an overseas Olympics, after Rio (67 medals).
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  335. momo says:

    Svaneti is a sparsely populated landscape roughly the size of Devon, England, that’s marked by snow-capped peaks, alpine meadows and hundreds of now half-deserted villages. Throughout his childhood, Davit Khergiani spent each summer here, in his family’s native Mestia, the small provincial capital that doubles as the starting point of the Mestia-to-Ushguli trail. His family have a traditional koshki, one of the roughly 175 medieval defensive stone towers built by the autonomous Svan clans who have traditionally governed this region with little outside interference.
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  336. nongnee says:

    Most days after class, I would swap my school shoes for mud-encrusted wellies, watching with envy as my friends were whisked away in their parents’ warm, dry cars. My mum was working long hours in the city and there were no public buses plying the school’s narrow access roads, so I had two options: walk the two-mile bridleway that connected my village to the school; or wait until dinnertime to be picked up. The decision, despite having to trudge through shin-deep mud for 40 minutes, was an easy one.
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  337. nongnee says:

    The government reported 71 new domestic cases on Wednesday – the highest daily count since January. A vaccination campaign for minors aged 12 to 17 is being expanded to try and combat the spread of the highly contagious delta variant. The latest cases have now spread to at least 16 provinces and municipalities It is unclear how many in China are fully vaccinated, although authorities say more than 1.6 billion doses have been administered so far.
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  338. Michel says:

    The midfielder – who joins on a six-year contract – is expected to make his Premier League debut for City at Tottenham on Sunday.Grealish set out several reasons for wanting to join City, including his hope it would boost his chances of a starting spot with England, and his desire to play under manager Pep Guardiola.He said playing in the Champions League and “performing” for the club “could set me up to have a brilliant future with the national team”. Grealish impressed for England in Euros warm-up wins against Austria and Romania and made one start and four substitute appearances, registering two assists, for England during the tournament.
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  339. Michel says:

    That was after the youngest player in the tournament – 19-year-old Will Smeed – smoked an unbeaten 65 off 38 balls as Phoenix smashed their way to 184-5, the highest score in the tournament.
    Smeed was ably supported by captain Moeen Ali, who made a blistering 28-ball 59 and hit three successive sixes off Graeme White, in front of a sell-out Edgbaston crowd of almost 18,000.
    Fire, who are now all-but eliminated, never got going in their reply – losing regular wickets throughout as they slipped to 91 all out from just 74 balls.
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  340. Michel says:

    The investment shows “how much we care about our priceless heritage of biodiversity and its enhancement in terms of sustainable tourism, especially in this post-Covid recovery period when we all feel the need to be more outdoors,” said Italy’s Minister of the Environment, Sergio Costa, when he announced the initiative.Conceived by a group of environmental journalists, the Grand Italian Route was completed in the 1990s but has been neglected in recent decades. Now, hikers, environmentalists and tourism officials are championing its new offshoot as a way to celebrate Italy’s rural soul and expand many travellers’ notions that the Italian landscape is limited to the rolling Tuscan countryside they see on postcards or screensavers.
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  341. Michel says:

    Most days after class, I would swap my school shoes for mud-encrusted wellies, watching with envy as my friends were whisked away in their parents’ warm, dry cars. My mum was working long hours in the city and there were no public buses plying the school’s narrow access roads, so I had two options: walk the two-mile bridleway that connected my village to the school; or wait until dinnertime to be picked up. The decision, despite having to trudge through shin-deep mud for 40 minutes, was an easy one.
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  342. Michel says:

    As I sat with my cappuccino and demi-baguette reading a collection of short stories by celebrated Rwandan writer Beata Umubyeyi Mairesse that I’d just found at Kigali’s best literary bookshop, Ikirezi Books, I noticed my phone was running low. I asked the young man at the next table, whom I’d overheard speaking English earlier (most people do in this trilingual city, but it’s good to check), if he had a charger. He did not. He went back to his MacBook, I to my short story, and then, about a minute later, he turned back to me.
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  343. AR Development Services focuses on 3D model design and implementation of the algorithms, responsible for overlaying the scene in the device’s camera lens with virtual content.

  344. momo says:

    It takes a lot to make Shelley Holroyd think twice about competing The javelin thrower once stopped a doctor from putting a plaster cast on her injured arm because it would have interfered with her Olympics training But if she were still competing, Holroyd is not sure she would feel comfortable taking part in the 2023 World Athletics Championships.
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  345. momo says:

    The event is being held in Hungary whose government has introduced a number of laws hostile to LGBTQ+ people – and Holroyd is gay Personally I’d be a little apprehensive about going she says If you’re an LGBTQ+ athlete and you’re going to a place that’s quite homophobic, you’re going to have that resting on your shoulders.
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  346. momo says:

    You know are they going to be booing you in the stadium If you get disrespected when you’re on the track or field, it’s not a good place to be, and it does affect people Holroyd will not have to make that decision, as she retired in 2006 – but an increasing number of active LGBTQ+ sportspeople are facing an unenviable choice.
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  347. nongnee says:

    Do they compete in countries with laws or governments that discriminate against LGBTQ people or stay at home and miss competitions they ve spent a lifetime training for Governing bodies too are under mounting pressure to explain how awarding events to such countries fits with their own commitments to inclusion and diversity.
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  348. nongnee says:

    The sporting calendar for the next few years shows just how many major events are being held in countries with laws hostile to the LGBTQ community Oman – where homosexuality is illegal – has hosting rights for the 2024 Hockey5s World Cup – a five-a-side version of the sport – and will co-host the Men’s T20 Cricket World Cup this October and November.
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  349. France says:

    The team had gone through the rural village of were only 40km from home when all of a sudden, after making a turn, they found themselves surrounded by a wildfire. It was a scene of complete pandemonium; parts of flaming trees flew through the air with a noise that sounded like the roaring sea. Redondo couldn’t believe his eyes. He told the driver Luis Chaves to stop immediately. Chaves however, didn’t listen to him. The bus was too big to quickly turn around, he thought – it was too dangerous. He kept saying: “I have to go through it, I have to go through it.
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  350. France says:

    The offers came in. Following further success at the 1992 World Junior Championships in Seoul, South Korea, where he won silver in the 100m and 200m as well as gold in the 4x100m relay, Campbell was in demand. He eventually decided to go and train with Colin Jackson’s coach, Malcolm Arnold, in Newport, south Wales. Campbell initially lodged with Arnold’s family as he made the transition from junior track star to full-time athlete. But while he looks back on those times fondly with a mentor he describes as “the best coach in the world”, the young Mancunian did not immediately take to a life in athletics.
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  351. mopmo says:

    Many elite athletes will list communication as a key attribute for success – which is why some people with stammers might think that team sport isn’t for them.
    But in reaching the top of their profession, both Brown and Shell say sport has actually helped their stammers. They have found that on the field, stammering was never an issue. For Brown, he puts that down to the team environment and to the quality of his team-mates.
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  352. mopmo says:

    Research from the BBC Afghan service shows the militants now have a strong presence across the country, including in the north and north-east and central provinces like Maidan  They are also closing  in the west, and the southern cities of Kandahar and Lashkar . US troops and their allies forced the Taliban from power in November 2001. The group had been Osama Bin Laden and other al-Qaeda figures linked to the 11 September 2001 attacks in the US.
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  353. mopmo says:

    As well as increased general stress and anxiety levels, a rise in sleep problems has been put down to other factors caused by the pandemic – such as the enforced changes to daily routines and social lives. With tired staff less productive, the pandemic has led to a number of companies focusing on helping their employees get a better quantity, and quality, of sleep. Ms Baker’s employer, a London-based marketing firm called MVF, is one such business.
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  356. Rosey says:

    Despite there being 41 full-time professionals in the women’s game, Windsor is one of the many players balancing multiple careers with dreams of becoming a professional cricketer.
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  357. Rosey says:

    Longlisted for the 2021 Booker Prize, this New York Times bestseller weaves together the stories of two women living decades and continents apart: Marian Graves, an intrepid pilot whose plane goes missing while she attempts to circle the globe, and Hadley Baxter, a scandal-racked Hollywood actress recently sacked from a Twilight-alike movie franchise, who is drawn to Graves’s story.
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  358. Rosey says:

    In his past wresting life, Johnson, then better known as The Rock, followed in the footsteps of his father, the pioneering Rocky Johnson, who along with partner Tony Atlas, was the first black champion in WWE history when the duo became tag team champs in 1983. His son then made his WWE debut in 1996 and stopped wrestling fulltime in 2004 as his movie career
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  359. nongnee says:

    Bautista has turned his overly-serious wrestling persona on its head and slid effortlessly into being the kind of funny personality that defies expectations. In the Guardians of the Galaxy movies, he has turned the green-skinned Marvel superhero Drax the Destroyer into something more than a generic CGI heavy with his sardonically witty line readings.
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  361. momo says:

    If you’ve ever watched the classic 1989 American film Field of Dreams, starring Kevin Costner, then prepare for the goosebumps you are about to experience.On Thursday, Major League Baseball staged a match in a cornfield, with Costner leading an epic pre-match entrance through the corn.Field of Dreams sees an Iowa corn farmer build a baseball diamond on his land after hearing a voice saying “if you build it, they will come”.
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  362. momo says:

    The Field of Dreams game between the Chicago White Sox and the New York Yankees has been planned for a number of years, but because of the coronavirus pandemic and logistical challenges it was postponed until now.Costner addressed the 8,000-strong crowd on an old-fashioned microphone before the first pitch of the first MLB game to be played in Iowa.Thirty years ago, on the other side of that corn, we filmed a movie that stood the test of time,” he said.
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  363. nongnee says:

    Tonight, thanks to that enduring impact that little movie had, it’s allowed us to come here again.We’ve come to see the first-place White Sox play the mighty Yankees in a field that was once corn. It’s perfect. We’ve kept our promise, MLB has kept its promise. The dream is still alive.”Referencing a well-known line from the film, he added: “There’s probably just one question to answer: Is this heaven? Yes, it is. This field is for the players. Good luck today.”The spectacle delighted the crowds, and the actual game had a Hollywood-style ending, too.
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  364. momo says:

    Brentford’s Thomas Frank made his entrance as a Premier League manager with an animated arm-waving plea for noise and support from those inside the vibrant Brentford Community Stadium who had waited so long to savour a night like this.The charismatic Dane, a cult figure loved by Brentford fans after ending their 74-year wait for top-tier status, need not have bothered.Brentford’s followers needed no encouragement or orchestration
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  365. nono says:

    The stage was set hours before kick-off as fans, finally allowed to fill this modern stadium to a 16,479 capacity, gathered around Kew Bridge Station and Chiswick roundabout with a sense of anticipation that was almost tangible.And expectations were fulfilled in 90 minutes Brentford fans will never forget, ending with the chant of “we’re top of the league” (which of course, they are) after a fully merited 2-0 win over Arsenal.
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