Figure 1: Cas9 Nickase has 1 inactive nuclease domaine to induce specific single-strand DNA nicks (original figure)




Nuclease domains of the Cas9 nuclease may be mutated independently of each other to create DNA “nickases” capable of introducing a single-strand cut with the same specificity as a regular CRISPR/Cas9 nuclease (Gasiunas et al. 2012).  The use of Cas9 nickases is essentially the same as the use of the fully functional enzyme.


Dual Nickases

Figure 2: Multiplexing of Cas9 Nickases is used to induce double strand breaks with overhangs with significantly reduced off-target effects (original figure)

Double-strand breaks can be introduced through the use of paired nickases for cooperative genome engineering  (Mali et al. Aug 2013).  A major difference for this approach is that, when two Cas9 nickases are used, long overhangs are produced on each of the cleaved ends instead of blunt ends.  This provides even greater control over precise gene integration and insertion (Mali et al. Sept 2013). Because both nicking Cas9 enzymes must effectively nick their target DNA, paired nickases have significantly lower off-target effects compared to the double-strand-cleaving Cas9 system, and are generally more effective tools (Ran et al. 2013; Mali et al. Aug 2013).



82 Responses to Nickases

  1. Susanna says:

    I love this website, and I have used this as reference for over a year now. Do you think it is possible to add a tab, showing how cytidine deaminase interacts with the target dna? I know it has something to do with a zinc finger, but what part of the dna/rna is the zinc finger bonding with? The PAM section? The nucleic acid before or after the PAM section? The target dna? How is cytidine deaminase bonding with CRISPR Cas9? This field fascinates me.

  2. suba suba says:

    phl0kc Terrific Post.thanks for share..much more wait..

  3. Zianezoz says:

    Hi, I am the best top online casino web developer,!

  4. ferahtia_FS says:

    very good article thanks for sharing

  5. GSBrental says:

    Excellent weblog here! Also your website loads
    up very fast! What web host are you the use of?

    Can I get your associate hyperlink in your host?
    I desire my site loaded up as quickly as yours lol

  6. It was very useful and informative

  7. Abdelmuhaimen Omar says:


  8. RACK1 says:

    atga ctgagcagat gacccttcgt ggcaccctca agggccacaa cggctgggta acccagatcg ctactacccc gcagttcccg gacatgatcc tctccgcctc tcgagataag accatcatca tgtggaaact gaccagggat gagaccaact atggaattcc acagcgtgct ctgcggggtc actcccactt tgttagtgat gtggttatct cctcagatgg ccagtttgcc ctctcaggct cctgggatgg aaccctgcgc ctctgggatc tcacaacggg caccaccacg aggcgatttg tgggccatac caaggatgtg ctgagtgtgg ccttctcctc tgacaaccgg cagattgtct ctggatctcg agataaaacc atcaagctat ggaataccct gggtgtgtgc aaatacactg tccaggatga gagccactca gagtgggtgt cttgtgtccg cttctcgccc aacagcagca accctatcat cgtctcctgt ggctgggaca agctggtcaa ggtatggaac ctggctaact gcaagctgaa gaccaaccac attggccaca caggctatct gaacacggtg actgtctctc cagatggatc cctctgtgct tctggaggca aggatggcca ggccatgtta tgggatctca acgaaggcaa acacctttac acgctagatg gtggggacat catcaacgcc ctgtgcttca gccctaaccg ctactggctg tgtgctgcca caggccccag catcaagatc tgggatttag agggaaagat cattgtagat gaactgaagc aagaagttat cagtaccagc agcaaggcag aaccacccca gtgcacctcc ctggcctggt ctgctgatgg ccagactctg tttgctggct acacggacaa cctggtgcga gtgtggcagg tgaccattgg cacacgctag

  9. Pros near me says:

    I see that post professional! Very thanks for content!

  10. jack nelson says:

    Thanks for the important information. Genetic engineering is important and complex science. I study biotechnology and study bio- and genetic engineering to understand the nature of mutations and then use my knowledge to develop cures for incurable diseases. Now I am writing a scientific work on the types of mutations and looking for unique information. I recently found a literature review service, professionals will be able to find the information I need and help me write my project.

  11. Robert Weeks says:

    This Was An Amazing ! I Haven’t Seen This Type of Blog Ever ! Thankyou For Sharing,

  12. أسهل طريقة لمعرفة اتجاه القبلة في المنزل. استخدم الخرائط على الإنترنت للعثور على القبلة.

  13. Billie says:

    Yeah, i am checking it.

  14. kledo5 says:

    Trying to make music often, even though I still can’t afford it Drift Hunters

  15. Nice information with an epic information,,,,,You can take different ideas frim different things which are available. Also take idea from https//

  16. Download New Music BY : Hojat Ashrafzade | Asheghe Toam With Text And 2 Quality 320 And 128 On Music-fa

  17. jolia says:

    Download New Music BY : Babak Jahanbakhsh | Az Khoshi Mimiram With Text And 2 Quality 320 And 128 On Music-fa download music

  18. taskmiran says:

    Xiaomi phone is the best brand ever!

  19. الهام چرخنده She is one of the well-known Iranian actors who has distanced herself from acting and people

  20. خرید محصولات شرکت بیز از هوپینو شاپ

  21. music says:

    very nice post

  22. cladder says:

    The article is very interesting!

  23. mark says:

    if you want to bet on legit sports betting click this okbet login

  24. novelwebsolution says:

    You have made some good points there. It provides me great pleasure to read your posts.

    Shopify Web Development Company In Jaipur

  25. harivansh tours says:

    A must-read post! Good way of describing and pleasure piece of writing. Thanks!

    Best Travel Agency in Jaipur

  26. Subhash Shastri says:

    People who are struggling in their life, your article will be proven helpful for them. They must read this article.
    Free Astrology Consultancy Advice Online

  27. artic says:

    Thanks, your article was really me site

  28. play snake says:

    ​Really I appreciate the effort you made to share the knowledge. The topic here I found was really effective to the topic which I was researching for a long time.

  29. Tommy Curlan says:

    get know about the latest one klima dao price

  30. Rosie Nelson says:

    my knowledge to develop cures for incurable diseases. Now I am writing scientific work on the types of mutations and looking for unique information.take my class

  31. Rachel Catt says:

    I have been a long-time customer of the website. I would love to see the site add a tab that shows how cytidine deaminase interacts with the target dna.

  32. Alex Morgan says:

    Book Marketing Services: Book Marketing Services is a book marketing company that offers a wide range of services to authors and publishers. From copywriting and editing to marketing, Book Marketing Services has you covered. Services offered by Book Marketing Services include: – Copywriting and editing – Book marketing – Social media marketing – Book design – Website design – Email marketing – Content creation – Search engine optimization – Social media management – Social media analytics – Paid search marketing – Digital marketing – Search engine optimization Book Marketing Services

  33. Ayaan Mishra says:

    We are the Best Affiliate Marketing Company Delhi. Being a Top Affiliate Marketing Agency we offer affordable Affiliate Marketing Services in India to increase the traffic on your website.

  34. Double-strand breaks can be introduced through the use of paired nickases for cooperative genome engineering

  35. This provides even greater control over precise gene integration and insertion (Mali et al. Sept 2013). Because both nicking Cas9 enzymes must effectively nick their target DNA

  36. What an awesome post, I just read it from start to end your blog post, An informative blog.

  37. Happy wheels says:

    I’d like to learn more about this issue because it’s fascinating. If you’re familiar with vintage games, Come play my game.

  38. Ayushi Bosus says:

    we will always be grateful to you and will share your hard-written article with our friends too.

  39. Mumbai says:

    Nice Post Good Work

  40. superedan68 says:

    The winner of the game is the one who can merge two matching numbers with their fingers the fastest. 1v1 lol

  41. Your website has a lot of valuable content. Good luck

  42. Daniel Wilson says:

    I want to buy a new mouse. I like the shape of the mouse and the color, but it has some major issues. The mouse constantly lags, the scroll wheel doesn’t work, and it makes clicking noises when I’m trying to type. The role of the teacher in a student’s life is significant. They make the students’ lives better by producing good skills in students’ personalities. When students get some positive feedback from their teachers, they encourage to perform well in their studies. You can also select this topic for your research paper. In online classes, students have no physical communication with their teachers. Most of the students search on the internet pay to take my online class. You can also do your research on this topic. These are some research topics on which you can easily find content to do your research in the best way

  43. Albert Costello says:

    This year for Christmas, women should look for jackets with unique designs and colors. You should buy this Christmas Womens Red Leather Jacket because it will not only keep your body comfortable but also make you stand out.

Leave a Reply

Your email address will not be published.

Looking for something?

Use the form below to search the site:

Still not finding what you're looking for? Drop a comment on a post or contact us so we can take care of it!

Visit our friends!

A few highly recommended friends...


    All entries, chronologically...

    Set your Twitter account name in your settings to use the TwitterBar Section.