Publications

Peer-reviewed journal articles

  1. Brown, A., Stoukides, D.M., Tzanakakis, E.S., Quantifying human pluripotent stem cell attributes with population balance modeling, 2024 (submitted).
  2. Ahuja, V., Jeyabalan, S., Tzanakakis, E.S., Assessment of pancreatic beta- and alpha-cell transduction by adeno-associated virus serotypes 2, 6 and 9, 2024 (submitted).
  3. Bolunduro, O.A., Chen, Z., Fucetola, C., Lai, Y.-R., Cote, M., Kajola, R.O., Rao, A.A., Liu, H., Tzanakakis, E.S., Timko, B.P., An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function, Adv. Sci., 11:e2402236, 2024.
  4. Chen, Z., Stoukides, D.M., Tzanakakis, E.S., Light-mediated enhancement of glucose-stimulated insulin release of optogenetically engineered human pancreatic beta-cells, ACS Synth. Biol., 13:825-36, 2024.
  5. Brown, A., Tzanakakis, E.S., Mathematical modeling clarifies the paracrine roles of insulin and glucagon on the glucose-stimulated hormonal secretion of pancreatic alpha-and beta-cells, Front. Endocrinol., 14, 2023.
  6. Chen, Z., Truskinovsky, L., Tzanakakis, E.S., Emerging molecular technologies for light-mediated modulation of pancreatic beta-cell function, Mol. Metab., 64:101552, 2022.
  7. Ashok, P., Tzanakakis, E.S., Proteomic analysis of exosomes during cardiogenic differentiation of human pluripotent stem cells, Cells, 10(10):2622, 2021.
  8. Jacobson, E.F., Chen, Z., Stoukides, D.M., Nair, G.G., Hebrok, M., Tzanakakis, E.S., Non-xenogeneic expansion and definitive endoderm differentiation of human pluripotent stem cells in an automated bioreactor, Biotechnol. Bioeng., 118(2):979-91, 2021.
  9. Ashok, P., Parikh, A., Du, C., Tzanakakis, E.S., Xenogeneic-free system for biomanufacturing of cardiomyocyte progeny from human pluripotent stem cells, Front. Bioeng. Biotechnol., 8:571425, 2020.
  10. Nair, G.G., Tzanakakis, E.S., Hebrok, M., Emerging routes to the generation of functional β-cells for diabetes mellitus cell therapy, Nat. Rev. Endocrinol., 16(9):506-18, 2020.
  11. Chen, Z., Downing, S., Tzanakakis, E.S., Four decades after the discovery of regenerating islet-derived (Reg) proteins: Current understanding and challenges, Front. Cell Dev. Biol., 7:235, 2019.
  12. Zhang, F., Tzanakakis, E.S., Amelioration of diabetes in a murine model upon transplantation of pancreatic β-cells with optogenetic control of cyclic AMP, ACS Synth. Biol., 8:2248-55, 2019.
  13. Downing, S., Zhang, F., Chen, Z., Tzanakakis, E.S., MicroRNA-7 directly targets Reg1 in pancreatic cells, Am. J. Physiol. Cell Physiol., 317(2):C366-C374, 2019.
  14. Jacobson, E.F., Tzanakakis, E.S., Who will win: Induced pluripotent stem cells versus embryonic stem cells for β-cell replacement and diabetes disease modeling?, Curr. Diab. Rep., 18:133, 2018.
  15. Zhang, F., Tzanakakis, E.S., Optogenetic regulation of insulin secretion in pancreatic β-cells, Sci. Rep., 7(1):9357, 2017.
  16. Jacobson, E.F., Tzanakakis, E.S., Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions, J. Biol. Eng., 11:21, 2017.
  17. Fan, Y., Zhang, F., Tzanakakis, E.S., Engineering xeno-free microcarriers with recombinant vitronectin, albumin and UV irradiation for human pluripotent stem cell bioprocessing, ACS Biomater. Sci. Eng., 3(8):1510-18, 2017.
  18. Ashok, P., Fan, Y., Rostami, M.R., Tzanakakis, E.S., Aggregate and microcarrier cultures of human pluripotent stem cells in stirred-suspension systems, Methods Mol. Biol., 1502:35-52,2016.
  19. Rostami, M.R., Wu, J., Tzanakakis, E.S., Inverse problem analysis of pluripotent stem cell aggregation dynamics in stirred-suspension cultures, J. Biotechnol., 208:70-9, 2015.
  20. Parikh, A., Wu, J., Blanton, R., Tzanakakis, E.S., Signaling pathways and gene regulatory networks in cardiomyocyte differentiation, Tissue Eng. Part B Rev., 21(4):377-92, 2015.
  21. Terranova, C., Narla, S.T., Lee, Y-W., Bard, J., Parikh, A., Stachowiak, E.K., Tzanakakis, E.S., Buck, M.J., Birkaya, B., Stachowiak, M.K., Global developmental gene programming involves a nuclear form of Fibroblast Growth Factor Receptor-1 (FGFR1), PLOS One, 10(4):e0123380, 2015.
  22. Wu, J., Fan, Y., Tzanakakis, E.S., Increased culture density is linked to decelerated proliferation, prolonged G1 phase and enhanced propensity for differentiation of self-renewing human pluripotent stem cells, Stem Cells Dev., 24(7):892-903, 2015.
  23. Fan, Y., Wu, J., Ashok, P., Hsiung, M., Tzanakakis, E.S., “Production of human pluripotent stem cell therapeutics under defined xeno-free conditions: Progress and challenges“, Stem Cell Rev., 11(1):96-109, 2015.
  24. Wu, J., Rostami, M.R., Cadavid Olaya, D.P., Tzanakakis, E.S., Oxygen transport and stem cell aggregation in stirred-suspension bioreactor cultures, PLOS One, 9(7):e102486, 2014.
  25. Fan, Y., Hsiung, M., Cheng, C., Tzanakakis, E.S., Facile engineering of xeno-free microcarriers for the scalable cultivation of human pluripotent stem cells in stirred suspension, Tissue Eng., 20(3-4):588-99, 2014.
  26. Chen, C.K., Law, W.C., Aalinkeel, R., Yu, Y., Nair, B., Wu, J., Mahajan, S., Reynolds, J.L., Li, Y., Lai, C.K., Tzanakakis, E.S., Schwartz, S.A., Prasad, P.N., Cheng, C., Biodegradable cationic polymer nanocapsules for overcoming multidrug resistance and enabling drug-gene co-delivery to cancer cells, Nanoscale, 6(3):1567-72, 2014.
  27. Tzanakakis, E.S., Hu, W.S., Stem cell science has made major strides in the last few years. Introduction., Biotechnol. Adv., 31(7): 993, 2013.
  28. Wu, J., Tzanakakis, E.S., Deconstructing stem cell population heterogeneity: Single-cell analysis and modeling approaches, Biotechnol. Adv., 31(7): 1047-62, 2013.
  29. Bett, G.C.L., Kaplan, A.D., Lis, A., Cimato, T., Tzanakakis, E.S., Zhou, Q., Morales, M.J., Rasmusson, R.L., Electronic ‘expression’ of the inward rectifier in cardiomyocytes derived from human induced pluripotent stem cells, Heart Rhythm 10(12):1903-10, 2013.
  30. Wu, J., Tzanakakis, E.S., Distinct allelic patterns of Nanog expression impart embryonic stem cell population heterogeneity, PLOS Comp. Biol., 9(7):e1003140, 2013.
  31. Wu, J., Rostami, M.R., Tzanakakis, E.S., Stem cell modeling: from gene networks to cell populations,Curr. Opin. Chem. Eng., 2(1):17-25, 2013.
  32. Mann, J.K., Wood, J.F., Stephan, A.F., Tzanakakis, E.S., Ferkey, D.M., Park, S., Epitope guided engineering of protein binders for in vivo inhibition of ERK-2 signaling, ACS Chem. Biol., 8(3):608-16, 2013.
  33. Stachowiak, M.K., Kucinski, A., Curl, R., Syposs, C., Yang, Y., Narla, S., Terranova, C., Prokop, D., Klejbor, I., Bencherif, M., Birkaya, B., Corso, B., Parikh, A., Tzanakakis, E.S., Wersinger, S., Stachowiak, E.K., Schizophrenia: A neurodevelopmental disorder – integrative genomic hypothesis and therapeutic implications from a transgenic mouse model, Schizophrenia Research, 143(2-3):367-76, 2013.
  34. Wu, J., Tzanakakis, E.S., Contribution of stochastic partitioning at human embryonic stem cell division to NANOG heterogeneity, PLoS ONE, 7(11):e50715, 2012.
  35. Lee, Y.W., Terranova, C., Birkaya, B., Narla, S., Kehoe, D., Parikh, A., Dong, S., Ratzka, A., Brinkmann, H., Aletta, J.M., Tzanakakis, E.S., Stachowiak, E.K., Claus, P., Stachowiak, M.K., A novel nuclear FGF Receptor-1 partnership with retinoid and Nur receptors during gene programming of embryonic stem cells, J. Cell Biochem., 113(9):2920-36, 2012.
  36. Patil, S.A., Chandrasekaran, E.V., Matta, K.L., Parikh, A., Tzanakakis, E.S., Neelamegham, S., Scaling down the size and increasing the throughput of glycosyltransferase assays: Activity changes on stem cell differentiation, Anal. Biochem., 425(2):135-144, 2012.
  37. Parikh, A., Stephan, A.F., Tzanakakis, E.S., Regenerating proteins and their expression, regulation and signaling, Biomol. Concepts, 3(1):57-70, 2012.
  38. Lock, L.T., Laychock, S.G., Tzanakakis, E.S., Pseudoislets in stirred-suspension culture exhibit enhanced cell survival, propagation and insulin secretion, J. Biotechnol., 151(3):278-86, 2011.
  39. Jing, D., Parikh, A., Tzanakakis, E.S., Cardiac Cell Generation from Encapsulated Embryonic Stem Cells in Static and Scalable Culture Systems, Cell Transplant., 19(11):1397-1412, 2010.
  40. Jing, D., Kehoe, D.E., Tzanakakis, E.S., Expression of Reg Family Proteins in Embryonic Stem Cells and its Modulation by Wnt/β-catenin signaling, Stem Cells Dev., 19(9):1307-19, 2010.
  41. Kehoe, D.E., Jing, D., Lock, L.T., Tzanakakis, E.S., Scalable Stirred-suspension Bioreactor Culture of Human Pluripotent Stem Cells, Tissue Eng. Part A, 16(2):405-21, 2010.
  42. Lock, L.T., Tzanakakis, E.S., Expansion and differentiation of human embryonic stem cells to endoderm progeny in a microcarrier stirred-suspension culture, Tissue Eng. Part A, 15(8):2051-63, 2009.
  43. Jing, D., Parikh, A., Canty, J.M., Tzanakakis, E.S., Stem cells for heart cell therapies, Tissue Eng. Part B, 14(4):393-406, 2008.
  44. Kehoe, D.E., Lock. L.T., Parikh, A., Tzanakakis, E.S., Propagation of embryonic stem cells in stirred suspension without serum, Biotechnol. Prog., 24(6):1342-52, 2008.
  45. Chemler*, J., Lock*, L.T., Koffas, M.A.G., Tzanakakis, E.S., Standardized Biosynthesis of Flavan-3-ols with Effects on Pancreatic Beta-Cell Insulin Secretion, Appl. Microbiol. Biotechnol., 77(4):797-807, 2007.*These authors have contributed equally.
  46. Lock, L.T., Tzanakakis, E.S., Stem/progenitor cell sources of insulin-producing cells for the treatment of diabetes, Tissue Eng., 13(7):1399-1412, 2007.
  47. Kawahira, H., Ma, N.H., Tzanakakis, E.S., McMahon, A.P., Chuang, P.T., Hebrok, M., “Combined Activities of Hedgehog Signaling Inhibitors Regulate Pancreas Development“, Development,130(20):4871-79, 2003.
  48. Tzanakakis, E.S., Waxman, D.J., Hansen, L.K., Remmel, R.P., Hu, W.S., Long-term Enhancement of CYP2B1/2 Expression in Rat Hepatocyte Spheroids through Adenovirus-mediated Gene Transfer, Cell Biol. Toxicol., 18:13-27, 2002.
  49. Tzanakakis, E.S., Hsiao, C.C., Matsushita, T., Remmel, R.P., Hu, W.S., Probing Enhanced Cytochrome P450 2B1/2 Activity in Rat Hepatocyte Spheroids through Confocal Laser Scanning Microscopy, Cell Transplant., 10(3):329-342, 2001.
  50. Tzanakakis, E.S., Hansen, L.K., Hu, W.S., The Role of Actin Filaments and Microtubules in Hepatocyte Spheroid Self-Assembly, Cell Motil. Cytoskeleton, 48(3):175-189, 2001.
  51. Tzanakakis, E.S., Hess, D.J., Sielaff, T.D., Hu, W.S., Extracorporeal Tissue Engineered Liver-Assist Devices, Annu. Rev. Biomed. Engr., 2:607-632, 2000.

Books edited

  • Stem Cells – From Mechanisms to Technologies, Editors: Stachowiak, M.K. and Tzanakakis, E.S., World Scientific Publishing, 2011.

Book chapters

  1. “Scalable expansion of human pluripotent stem cells for biomanufacturing cellular therapeutics”, Stoukides, D., Jacobson, E.F., Tzanakakis, E.S., in Advances in Stem Cell Biology, volume Induced Pluripotent Stem Cells – Novel Concepts, Editor: A. Birbrair, Elsevier, 2021
  2. “Large-scale culture of 3D aggregates of pluripotent stem cells”, Jacobson, E., Tzanakakis E.S., in The Gene and Cell Therapy series – Bioreactors for Stem Cell Expansion and Differentiation, Editors: J.M.S. Cabral, C.L. da Silva, CRC Press Taylor & Francis, 2018
  3. “Bioreactors and the design of the stem cell niche”, Fan, Y., Jing, D., and Tzanakakis E.S., in Stem Cell Biology and Regenerative Medicine series, Biology in Stem Cell Niche, Editor: K. Turksen, Springer, 2015
  4. “Stem Cell Bioprocessing for Regenerative Medicine”, Jing, D., Parikh, A. and Tzanakakis E.S., in Stem Cells – From Mechanisms to Technologies, edited by Stachowiak, M.K. and Tzanakakis, E.S., World Scientific Publishing, 2011
  5. “A Common Integrative Nuclear Signaling Module for Stem Cell Development”, Stachowiak, M.K., Stachowiak, E.K., Aletta, J.M. and Tzanakakis E.S., in Stem Cells – From Mechanisms to Technologies, Editors: Stachowiak, M.K. and Tzanakakis, E.S., World Scientific Publishing, 2011
  6. “Advances in Adult Stem Cell Culture,” Tzanakakis, E.S., Verfaillie, C.M., in Cell Culture Technology for Pharmaceutical and Cell-based Therapies, Editors: Ozturk, S., Hu, W.S., CRC Press, 2005

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