Animal Histology Core
Services provided by the Tufts Animal Histology Core
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Frozen Sectioning
- Preparation, freezing, and cutting of standard frozen sections
- Fixing, staining, and cover-slippin
- Note: Unsupervised investigator use of cryostat allowed following training
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Tissue Trimming
- Trimming of submitted fixed tissues to ensure best orientation for optimal assessment
- Decalcification of bone specimens
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Tissue Embedding
- Correct orientation of samples in paraffin wax
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Tissue Sectioning
- Basic microtomy to produce standard 5µm paraffin sections
- Advanced sectioning (e.g., step sectioning, levels, thick sections, etc.)
- Unstained sections in preparation for immunohistochemistry or other procedures
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Staining
- Standard H&E and special stains
- Rapid Hematoxylin and Eosin (H&E) for frozen sections
- Coverslip slides