Services provided by the Tufts Animal Histology Core

• Frozen Sectioning
  • Preparation, freezing, and cutting of standard frozen sections
  • Fixing, staining, and cover-slippin
  • Note: Unsupervised investigator use of cryostat allowed following training
• Tissue Trimming
  • Trimming of submitted fixed tissues to ensure best orientation for optimal assessment
  • Decalcification of bone specimens
• Tissue Embedding
  • Correct orientation of samples in paraffin wax
• Tissue Sectioning
  • Basic microtomy to produce standard 5µm paraffin sections
  • Advanced sectioning (e.g., step sectioning, levels, thick sections, etc.)
  • Unstained sections in preparation for immunohistochemistry or other procedures
• Staining
  • Standard H&E and special stains
  • Rapid Hematoxylin and Eosin (H&E) for frozen sections
  • Coverslip slides