Tag Archives: December2016

articles, December 2016, Techniques

Notes from Up North: What is an IDeA COBRE?

By Lucy Liaw, PhD Tufts/MMCRI

Here at Maine Medical Center Research Institute, we are very happy to be supporting Tufts trainees and working with many Tufts investigators here and in Boston to provide core facility services such as transgenic mouse generation.

Did you know that many of our core facilities were established at Maine Medical Center through a special NIH program, the Institutional Development Award (IDeA) Program? The IDeA program was established by Congressional mandate in 1993 to help develop research infrastructure to support biomedical research in 23 states that historically have had a low level of NIH funding. Maine is one of those states. In fact, there was a time when 50% of NIH funding went to researchers in 5 states (Massachusetts being one of those heavily funded states!), while the 23 IDeA eligible states together only received about 5% of all NIH funds. Over the last 23 years, NIH investment in biomedical research in Maine has contributed to a burgeoning biotech scene (http://www.mainebioscience.org/access_resources/bioscience-map-of-maine/) and a highly collaborative network of research institutes.

One of the components of the IDeA program is the Centers of Biomedical Research Excellence (COBRE). Maine Medical Center has been fortunate to have received two COBRE awards since 2000, one with the theme of Vascular Biology, and one in Stem and Progenitor Cell Biology. These awards have supported the recruitment of new junior investigators to Maine Medical Center (with appointments at Tufts University School of Medicine), and also the establishment and expansion of our core facilities.  Please visit our website at mmcri.org, and find “Core Facilities” under “Research Services & Resources” to see if we provide services that could be useful to your research!

Microinjection of mouse fertilized oocyte. Our Mouse Transgenic Facility performs genome modification using standard transgenesis, gene targeting in ES cells, or CRISPR/Cas. In 2017, we will start to offer services for CRISPR/Cas project design and sgRNA synthesis.
Microinjection of mouse fertilized oocyte. Our Mouse Transgenic Facility performs genome modification using standard transgenesis, gene targeting in ES cells, or CRISPR/Cas. In 2017, we will start to offer services for CRISPR/Cas project design and sgRNA synthesis.

 

Imaging by microCT. We run a Scanco vivaCT40 for microCT imaging of bone, teeth, fat, and the vasculature. Image, above right, shows microfil perfusion of the vasculature of a tumor xenograft, used to quantify and measure tumor angiogenesis.
Imaging by microCT. We run a Scanco vivaCT40 for microCT imaging of bone, teeth, fat, and the vasculature. Image, above right, shows microfil perfusion of the vasculature of a tumor xenograft, used to quantify and measure tumor angiogenesis.

 

Proteomics and Lipidomics Core Facility. We run a mass spectrometry resource with state-of-the-art protein and lipid profiling capacity. Recent studies include experiments to study tissues including adipose tissues and the skeleton, and how their protein and lipid content changes during metabolic disease.
Proteomics and Lipidomics Core Facility. We run a mass spectrometry resource with state-of-the-art protein and lipid profiling capacity. Recent studies include experiments to study tissues including adipose tissues and the skeleton, and how their protein and lipid content changes during metabolic disease.

 

Histopathology Core Facility. We provide full services for tissue processing, embedding, sectioning, routine histology, and immunostaining. We work closely with our Maine Medical Center Biobank to generate tissue arrays for screening of human disease specimens from patients.
Histopathology Core Facility. We provide full services for tissue processing, embedding, sectioning, routine histology, and immunostaining. We work closely with our Maine Medical Center Biobank to generate tissue arrays for screening of human disease specimens from patients.
December 2016, Library, PubMed Tip of the Month

PubMed Tip of the Month: My NCBI Preferences

You can customize your preferences to make searching PubMed and other NCBI databases easier.  Log in to your My NCBI account (see Insight March 2016 for details on creating a My NCBI account) and click the ‘NCBI Site Preferences’ link in the top right corner of the homepage.

Here are a few preferences that you may want to adjust:

  • Highlighting: Highlights your search terms in a set of results, making scanning for relevant articles more efficient.
  • Filters & Icons: Personalized filters displayed in the right-hand column on results page. I recommend adding the MEDLINE filter, which limits results to articles that have had MeSH terms applied to them.  To do so, click on the ‘Filters & Icons’ link on the Preferences page, and on the page that opens, select the ‘Properties’ radio button.  Enter ‘MEDLINE’ in the search with terms box, then check the box next to MEDLINE.  You can also add the ‘Find it @Tufts’ button, which enables you to access the full text of an article through Tufts Libraries.  Adding this button to your NCBI account would obviate the need to access PubMed through the Hirsh Health Sciences Library website.  To add the ‘Find it @Tufts’ button, select the ‘LinkOut’ radio button on the Filters & Icons page.  Then, enter ‘Tufts University’ in the search box and check the link icon box next to Tufts University Hirsh Health Sciences and Veterinary Libraries.
  • Outside Tool: A simpler method of adding the ‘Find it @Tufts’ button to your account preferences. Click the ‘Outside Tool’ link on the Preferences page.  On the page that opens, select the radio button next to ‘Tufts’.
  • Result Display Settings: You can choose the format (summary or abstract) in which results are displayed, how results are sorted (I do not recommend changing this from the default, recently added), and the number of items per page (I prefer 200, so I don’t have to click through multiple pages).

These are just a few of the preferences that you can adjust in your My NCBI account.  You can also explore customized settings for other NCBI databases, such as Gene.

Preferences page in My NCBI

Preferences page in My NCBI

December 2016, Library, On the Shelf

On the Shelf…

For Work…

 naturemicro_work_2016_12

Electronic resource: Nature Microbiology

Location: https://library.tufts.edu:443/record=b3035776~S1

One of the latest titles from Nature Publishing Group, this exclusively online journal is dedicated to research in the field of microbiology.

And leisure…

 jahrens_labgirl_leisure_2016_12

Lab Girl, Hope Jahren

Location: HHSL Leisure Reading, Sackler, 4th Floor, Non-Fiction J25 2016

Geobiologist’s memoir of her life in scientific research.

December 2016, Events for Students

GSC Committee & Club Updates: December 2016

Tufts Biomedical Business Club (TBBC)

from Aaron BernsteinCMP

Upcoming Events

TBBC Case Study Group: Mondays – 5-7PM beginning M Feb 6, Jaharis 508

Practice solving cases, gain insight and tips, and learn more about the field of consulting.

Recent Events

TBBC Seminar Series: Liz O’Day, Founder and CEO of Olaris Therapeutics

Tu Dec 6: Liz O’Day, PhD, presented actionable tips and insight into her transition from the academic world to being an entrepreneur. Olaris is a venture-backed drug discovery company that uses a proprietary NMR-metabolite profiling platform to unlock aspects of human metabolism that could never before be analyzed.

TBBC Consulting Seminar Series: Peter Bak, PhD

Tu Dec 13: Peter Bak, PhD, Manager at Back Bay Life Science Advisors, spoke about transitioning from a PhD program to life sciences consulting and career opportunities at BBLSA.

December 2016, Library, Notes from the library

Notes from the Library…Software on Library & Personal Computers

We often get asked about what statistical and data analysis programs are installed on the library’s computers, or available for installation on personal computers.  Here is a summary of the computers available at the Hirsh Health Sciences Library, and a chart indicating which statistical and data analysis programs are installed on these computers and available to students:

  • Public Computers: Desktop computers on the 4th and 5th floors of the Sackler; available for anyone to use.
  • Computers labs: Desktop computers in Sackler 510 and 514; available for use when not reserved for a class (check schedule on white board behind Tufts Technology Service Desk on 5th floor of Sackler). All computers in both labs were recently replaced.
  • Laptops: Mac and PC laptops available for checkout at the Library Service Desk on the 4th floor of Sackler; available for students, faculty and staff to checkout for 4 hours.

HHSLcomputers_statistics_software-ie-or8