Overview:

This week students will learn to propagate (passage/subculture) the isolated cells, as well as generate frozen stocks of the cells for storage in liquid nitrogen.

Assignments:

• Read Scaffold Fabrication Paper for next week

Lecture Slides:

Protocols:

[embeddoc url=”https://sites.tufts.edu/cellagcourse/files/2020/07/Wk4_Satellite_Cell_Passaging.docx” viewer=”microsoft”] [embeddoc url=”https://sites.tufts.edu/cellagcourse/files/2020/07/Wk4_Fat_Cell_Passaging.docx” viewer=”microsoft”] [embeddoc url=”https://sites.tufts.edu/cellagcourse/files/2020/07/Wk4_Satellite_Cell_FreezingThawing.docx” viewer=”microsoft”] [embeddoc url=”https://sites.tufts.edu/cellagcourse/files/2020/07/Wk4_Fat_Cell_FreezingThawing.docx” viewer=”microsoft”]