Week 3: Primary Bovine Cell Isolations

Overview/Goals for the week:

This week, practical laboratory learning commences with the very first step in the process of generating cultured meat – the initial isolation of stem cells from livestock. The class will take a field trip to the Tufts Veterinary School and collect small muscle/fat biopsies to transport back to the tissue culture laboratories. Back in the lab, we will process the tissue and select for muscle satellite cells and preadipocytes.

Assignments:

  • Select teams and brainstorm ideas for the final project

Lecture Slides:

Protocols: