Click here to download all files (slides and protocols): HERE
Module 1
Week 1 – Isolation Prep
Week-1-Isolation-PrepWeek 2 – Muscle and Fat Isolation
Week-2-Muscle-and-Fat-IsolationWeek 3 – Cell Passaging
Week-3-Cell-PassagingWeek 4 – Cryopreservation and muscle & fat differentiation
Week-4-Cryopreservation-and-muscle-^0-fat-differentiationWeek 5 – Verification of Differentiation
Week-5-Verification-of-Differentiation-1Module 2
Week 6 – DOE Media Design
Week-6-DOE-Media-DesignWeek 7 – Hydrogels
Week-7-HydrogelsWeek 8 – Hydrogels + ORO
Week-8-Hydrogels-and-OROWeek 9 – Mechanical Testing
Week-9-Mechanical-Testing-1Module 3
Week 10 – Antioxidant Treatments
Week-10-Antioxidant-TreatmentsWeek 11 – Viability Assay
Week-11-Viability-AssayWeek 12 – Cell Harvest
Week-12-Cell-HarvestWeek 13 – TBARS Assay
Week-13-TBARS-Assay