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Module 1

Week 1 – Isolation Prep

Week 2 – Muscle and Fat Isolation

Week 3 – Cell Passaging

Week 4 – Cryopreservation and muscle & fat differentiation

Week 5 – Verification of Differentiation

Module 2

Week 6 – DOE Media Design

Week 7 – Hydrogels

Week 8 – Hydrogels + ORO

Week 9 – Mechanical Testing

Module 3

Week 10 – Antioxidant Treatments

Week 11 – Viability Assay

Week 12 – Cell Harvest

Week 13 – TBARS Assay