The Prevalence of Antibiotic Resistance in the Environment (PARE) project began as a short-duration classroom project in which students cultured bacteria from local soil samples and assessed for colonies resistant to tetracycline (Core PARE module). Subsequently, the program has grown to include a library of additional short-term modules, all related to the core module. There is no required linear progression, however, the case study and molecular kit provide context for the other modules. Colony identification through the Core PARE module is required for the subsequent sub-culturing modules (Kirby-Bauer, Colony Identification, and Horizontal Transfer), which also require a biological safety cabinet and BioSafety level 2 training (not appropriate for high school students). The molecular modules (with the exception of the known outcome kit) require DNA isolation from a soil sample but no culturing of living organisms.
The PARE project uses a “modular” approach to classroom research. Most PARE classrooms implement the core PARE module, which is low cost and can be completed within 2-3 class periods. Instructors who wish to give their students a longer research experience can pick and choose from a library of additional short research modules, or choose to design and implement their own follow-up experiments.
To explore the modules, click here to begin with the Core PARE module.