Core PARE Module

Environmental prevalence of TcR

This is the flagship course-based research module of the Prevalence of Antibiotic-Resistant microbes in the Environment (PARE) program. It engages students in a crowdsourcing effort to provide environmental surveillance data that could lead to identification of “hotspots” or environmental resistance reservoirs. Students perform classic microbiological techniques such as serial dilution and plating, colony counting and colony-forming unit (CFU) calculations. Students are encouraged to create a hypothesis about collection sites that may harbor high or low levels of tetracycline resistance, although outcomes are completely unknown prior to class making this a true discovery project.

Module Organization

Process Overview

Instructional Videos


Instructor and Undergraduate Student Manual

Data Entry Instruction Slides

PARE Quick Reference

Calculations Worksheet for Nutrient Broth

Error-checking Spreadsheet Template

Curated Tetracycline Resistance Data through 2019

All files (.zip)