16S PCR from colony
Students choose a colony isolated from the core PARE module for tentative phylogenetic characterization. Genomic DNA is isolated and used in PCR reactions to amplify the 16S rRNA gene. Amplification results are confirmed by gel electrophoresis, the amplified DNA cleaned and sent for sequencing. Sequences are compared to those in the Ribosomal Database Project database to assign putative identity. Identity information is a critical early step to identify potential pathogens so this module is recommended prior to any future modules that require tetracycline-resistant colony sub-culturing
This module was developed in collaboration with Dr. Brad Goodner, Hiram College.