Identification of tetracycline-resistance genes from total soil DNA
Students test soil samples for the presence of different tetracycline-resistance genes. This module does not require handling of live organisms. Students extract genomic DNA directly from soil and subject to PCR followed by gel electrophoresis.
This module doesn’t require handling of biological organisms; however, the same methods can be applied to TcR colonies isolated from the core PARE module, which would require BSL-2 handling of environmental unknown organisms.
Development of this module is a collaborative effort with the miniPCR company and Dr. Adam Kleinschmit (University of Debuque) and Dr. Jennifer Larson (Capital University).