Tracing the course of antibiotic resistance using PCR

This lab represents a fictional case study of a very real problem. Students are presented with information regarding an outbreak of food-borne infection that is resistant to treatment with antibiotics. They are tasked with using PCR and gel electrophoresis to establish whether farms are at risk from antibiotic resistant bacteria that may be spreading in the environment. This known-outcome activity introduces students to the concept of environmental antibiotic resistance and to the molecular skills they will need for the non-culture-based research modules.

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This module is a collaborative effort with miniPCR and is appropriate for high school and undergraduate classrooms.