Kirby-Bauer testing TcR isolates
Students assess colonies isolated in the core PARE module for resistance to other antibiotics using the Kirby-Bauer disk diffusion assay which relies on measurement of the zone of inhibition after microbial growth around an antibiotic impregnated filter disk. Since the Kirby-Bauer test is intended for use on isolates of known identity, it is strongly suggested that students perform 16S tentative identification on test isolates prior to this module.
Module Organization
Acknowledgements
This module was developed in collaboration with Dr. Adam Kleinschmit, Dubuque University.