Environmental prevalence of TcR
This is the flagship course-based research module of the Prevalence of Antibiotic-Resistant microbes in the Environment (PARE) program. It engages students in a crowdsourcing effort to provide environmental surveillance data that could lead to identification of “hotspots” or environmental resistance reservoirs. Students perform classic microbiological techniques such as serial dilution and plating, colony counting and colony-forming unit (CFU) calculations. Students are encouraged to create a hypothesis about collection sites that may harbor high or low levels of tetracycline resistance, although outcomes are completely unknown prior to class making this a true discovery project.
Module Organization
Process Overview
Instructional Videos
Downloads
Instructor and Undergraduate Student Manual
Calculations Worksheet for Nutrient Broth
Error-checking Spreadsheet Template