Data Upload Instructions
Please review the Data Upload instructional slide deck prior to beginning any of the data-generating projects.
Category Archives: PARE
Protected: CFU/% Resistance
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Protected: eDNA/PCR
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Molecular-Based Modules
To build important molecular skills, we offer introductory and advanced modules where students can learn micropipetting, PCR and DNA extraction skills. These modules also avoid working with resistant unknown organisms, since work is done directly from soil without culturing. Videos in the Background section provide useful context for students. Tracing the course of antibiotic resistance
Culture-Based Modules
For a culture-based approach to surveillance, students start with the Core PARE Module in which soil is tested for the prevalence of live bacteria that are resistant to tetracycline and upload results to our database. From there, students in biosafety level 2-equipped laboratories can sub-culture resistant colonies and do further tests. 16S PCR from colony
PARE-Seq
Background videos produced by undergraduates provide students with the context necessary to understand the Galaxy-based bioinformatics activity. In the bioinformatics workflow, students analyze authentic soil and water metagenomic sequence files for the presence of antibiotic resistance genes. Meet the PARE-Seq undergraduate developers Background Videos It is recommended that students watch all five background videos here
Wet Lab
The following modules are all performed in the laboratory classroom (no online option available). Culture-Based Modules. For a culture-based approach to surveillance, students start with the Core PARE Module in which soil is tested for the prevalence of live bacteria that are resistant to tetracycline and upload results to our database. From there, students in
Skill-Building
The following videos provide how-to demonstrations for skills used in the PARE modules and background to provide context. How to Upload Data Build skills for the research modules This series of activities will help to prepare students to achieve more robust results with the authentic research modules. The activities include: How to use a micropipette